LOCUS       sequence-122836-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-PABPC1 M161A/D165K
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          163..295
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        340..358
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             370..756
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             763..804
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc
      121 ttaaggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      181 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      241 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc
      301 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct
      361 agcaccatgg cttctaactt tactcagttc gttctcgtgg acaatggcgg aactggcgac
      421 gtgactgtcg ccccaagcaa cttcgctaac ggggtcgctg aatggatcag ctctaactcg
      481 cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa
      541 tacaccatca aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg tgtagagctt
      601 cctgtagccg catggcgttc gtacttaaat atggaactaa ccattccaat tttcgccacg
      661 aactccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg
      721 attccctcag caatcgcagc aaactccggc atctacggcg gtggcaagcc catccctaac
      781 ccactgctgg gcctggacag caccctcgag aaccccagtg cccccagcta cccgatggcc
      841 tcgctctacg tgggggacct ccaccccgac gtgaccgagg cgatgctcta cgagaagttc
//
