LOCUS       sequence-122839-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PABPC1-MS2 fusion, double mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-PABPC1 MLLEmut M161A/D165K
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          167..299
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     282..301
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        344..362
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             374..760
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             767..808
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcacta gaagctttat tgcggtagtt tatcacagct
       61 taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagtgac
      121 tctcttaagg tagccttgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt
      181 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
      241 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
      301 tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg actcactata
      361 ggctagcacc atggcttcta actttactca gttcgttctc gtggacaatg gcggaactgg
      421 cgacgtgact gtcgccccaa gcaacttcgc taacggggtc gctgaatgga tcagctctaa
      481 ctcgcgttca caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg
      541 caaatacacc atcaaagtcg aggtgcctaa agtggcaacc cagactgttg gtggtgtaga
      601 gcttcctgta gccgcatggc gttcgtactt aaatatggaa ctaaccattc caattttcgc
      661 cacgaactcc gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa
      721 cccgattccc tcagcaatcg cagcaaactc cggcatctac ggcggtggca agcccatccc
      781 taacccactg ctgggcctgg acagcacccc tcgagaaccc
//
