LOCUS       sequence-122842-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Geph G domain expression in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Tyagarajan SK, Ghosh H, Yevenes GE, Imanishi SY, Zeilhofer HU, 
            Gerrits B, Fritschy JM
  TITLE     Extracellular signal-regulated kinase and glycogen synthase kinase 
            3beta regulate gephyrin postsynaptic aggregation and GABAergic 
            synaptic function in a calpain-dependent mechanism.
  JOURNAL   J Biol Chem. 2013 Apr 5;288(14):9634-47. doi: 
            10.1074/jbc.M112.442616. Epub 2013 Feb 13.
  PUBMED    23408424
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.442616"; journalName: "J Biol Chem"; date: 
            "2013-04-5- 5"; volume: "288"; issue: "14"; pages: "9634-47"
COMMENT     Sequence Label: pCR3-FLAG-Gephyrin G
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(615..662)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     primer_bind     complement(678..697)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(679..697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(742..793)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(771..791)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tagaataggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attccagcac actggcggcc gttactagtg gatccgagct cggtacctca aatggcatga
      121 ggtagagctg gcagtatgaa ctgaaagcat tcctgagatc ctttcttgct accaggcagg
      181 ttaattatca gagttttccc tcttattcca cacactggtc tagagagcat gcccagaggt
      241 gtaacattaa gtgatcccat cagcattgcc agggccatcc ctggcgcttc ccgttctatt
      301 acttcttttg tggcctctgg agtgacatct cgtggtgcaa accctgttcc gccagttgtt
      361 aatatcaaat taagttcctt ctcatcacac cagtctatga gtgtttcctt gatttcttct
      421 atttcatctg gtactatctt gtatgctgat atagtcccac ccaacaaaga aggatcctgg
      481 acgagatctt ttagatttat cccactgcgg tcttctgcaa gattcctgaa gcagctatca
      541 ctcactgtga ggactccgac acggatttga tggtcgtggt ttgtgaggat cattccctcg
      601 gtcgctcctg ctcccttgtc gtcgtcgtcc ttgtagtcct tgtcgtcgtc gtccttgtag
      661 tccataagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
      721 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
      781 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgatt
//
