LOCUS       sequence-122848-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: Cm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_4_Cm
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(625..631,632..649,650..655))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    670..691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(811..828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
       61 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
      121 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgggctcggg
      181 agacctatcg atctttctag aagatctcct acaatattct cagctgccat ggaaaatcga
      241 tgttcttctt ttattctctc aagattttca ggctgtatat taaaacttat attaagaact
      301 atgctaacca cctcatcagg aaccgttgta ggtggcgtgg gttttcttgg caatcgactc
      361 tcatgaaaac tacgagctaa atattcaata tgttcctctt gaccaacttt attctgcatt
      421 ttttttgaac gaggtttaga gcaagcttca ggaaactgag acaggaattt tattaaaaat
      481 ttaaattttg aagaaagttc agggttaata gcatccattt tttgctttgc aagttcctca
      541 gcattcttaa caaaagacgt ctcttttgac atgtttaaag tttaaacctc ctgtgtgaaa
      601 ttattatccg ctcataattc cacacattat acgagccgga agcataaagt gtaaagcctg
      661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc caattgcttt
      721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      841 tcggctgcgg cgagcggtat cagctcactc aa
//
