LOCUS       sequence-122850-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(137..817)
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(199..222)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(397..416)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctgcataac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 ctagagtcgc ggccgcttag gcgccggtgg agtggcggcc ctcggcgcgc tcgtactgtt
      181 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag cttgatgtcg gtcttgtagg
      241 cgccgggcag ctgcacgggc ttcttggcca tgtaggtggt cttgacctcg gcgtcgtagt
      301 ggccgccgtc cttcagcctc agcctcatct tcatctcgcc cttcagggcg ccgtcctcgg
      361 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
      421 cggaggggaa gttggtgccg cgcaccttca ccttgtagat gaactcgccg tcctgcaggg
      481 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
      541 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
      601 aggccttgga gccgtacatg atctgagggg acaggatgtc ccaggcgaag ggcagggggc
      661 cacccttggt caccttcagc ctggcggtct gggtgccctc gtaggggcgg ccctcgccct
      721 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgctcc ttgaagcgca
      781 tgaactcctt gatgacctcc tcgcccttgc tcaccatggt agccaccggt ggatccccgc
      841 tacctccggt gcctttgctt
//
