LOCUS       sequence-122851-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1113..1793
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1514..1533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1708..1731
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggatc tgcaagcctc gttgctgtcc actggcccca
       61 atgccagcaa catctccgat ggccaggata atttcacatt ggcggggcca cctcctcgca
      121 caaggagtgt ctcctacatc aacatcatca tgccttcagt gtttggtacc atctgtctcc
      181 tgggcattgt gggaaactcc acagtcattt ttgccgtggt gaagaaatcc aagctgcact
      241 ggtgcagcaa cgtccctgac atcttcatca tcaacctctc tgtggtggat ctgcttttcc
      301 tgctgggcat gcctttcatg atccaccagc tcatgggtaa tggtgtctgg cactttgggg
      361 aaaccatgtg caccctcatc acagccatgg acgccaacag tcagttcacc agcacctaca
      421 tcctgactgc tatggccatt gaccgctact tggccaccgt ccatcccatc tcctccacca
      481 agttccggaa gccctccatg gccaccctgg tgatctgcct cctgtgggct ctctcgttca
      541 ttagcatcac tcctgtgtgg ctctatgcca ggcttatccc cttcccaggg ggtgctgtgg
      601 gctgtggcat ccgcctacca aacccagata ctgatcttta ctggttcact ctgtatcagt
      661 ttttcctggc cttcgccctt ccgtttgtgg tcatcactgc tgcgtacgtg aaaatactac
      721 agcgcatgac gtcttcggtg gccccagcct ctcaacgcag catccggctt cggacaaaga
      781 gggtgacccg cacagccatt gccatctgtc tggtcttctt tgtgtgctgg gcgccctact
      841 acgtgctgca gctgacccag ttgtccatca gccgcccgac cctcacattc gtctacctgt
      901 acaatgcggc tatcagcttg ggctatgcca acagctgcct caatcccttt gtgtacatag
      961 tactctgtga gacctttcga aaacgcttgg tgctgtcggt gaagcccgcg gcccaggggc
     1021 agcttcgcac ggtcagcaat gctcagacag ctgacgagga gaggacagaa agcaaaggca
     1081 ccggaggtag cggggatcca ccggtggcta ccatggtgag caagggcgag gaggtcatca
     1141 aggagttcat gcgcttcaag gagcacatgg agggctccgt gaacggccac gagttcgaga
     1201 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccagg ctgaaggtga
     1261 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagatc atgtacggct
     1321 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     1381 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     1441 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact
     1501 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     1561 ggatgtaccc cgaggacggc gccctgaagg gcgagatgaa gatgaggctg aggctgaagg
     1621 acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc
     1681 tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca
     1741 ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc taagcggccg
     1801 cgactctaga tcataatcag ccataccaca tt
//
