LOCUS       sequence-122855-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Yamamoto A, Schofield MJ, Biswas I, Hsieh P
  TITLE     Requirement for Phe36 for DNA binding and mismatch repair by 
            Escherichia coli MutS protein.
  JOURNAL   Nucleic Acids Res. 2000 Sep 15. 28(18):3564-9.
  PUBMED    10982877
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2000 Sep 15. 28(18):3564-9."
COMMENT     Sequence Label: pAY02
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             648..659
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgccgcg cggcagccat atgagtgcaa tagaaaattt cgacgcccat acgcccatga
      121 tgcagcagta tctcaggctg aaagcccagc atcccgagat cctgctgttt taccggatgg
      181 gtgatgctta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg ctggatattt
      241 cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg attccctacc
      301 atgcggtgga aaactatctc gccaaactgg tgaatcaggg agagtccgtt gccatctgcg
      361 aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg
      421 ttacgccagg caccatcagc gatgaagccc tgttgcagga gcgtcaggac aacctgctgg
      481 cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc agttccgggc
      541 gttttcgcct gagcgaaccg gctgaccgcg aaacgatggc ggcagaactg caacgcacta
      601 atcctgcgga actgctgtat gcagaagatt ttgctgaaat gtcgttaatt gaaggccgtc
      661 gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga
      721 atctgc
//
