LOCUS       sequence-122866-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged 
            nlsTdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pTol1-uas-mAKT-2a-nlstdTomato
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    208..228
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     230..249
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        230..248
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     273..289
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     274..290
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(640..656)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(640..656)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(693..711)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    711..735
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             749..769
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 tctaggccgg gccgtccggg cggtggcctg gggcggaaaa ctgaaggggg gcggcaccgg
       61 cggctcagcc ctttgtaata tattaatatg caccactatt ggtttactta tgtcacagtt
      121 tgtaagtttg taacagcctg aacctggccg cgcgccattg ggatggaacc ctcgaggggc
      181 ccgctagcga tatccctgca gggctatcaa ctttgtatag aaaagttggt aatacgactc
      241 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttag
      301 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg
      361 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg
      421 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag
      481 aggtcggagt actgtcctcc gacgcaaggc ggagtactgt cctccgggct ggcggagtac
      541 tgtcctccgg caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct
      601 cactttgagc tcctccacac gaattcctgc agcccggggg atccactagt tctagagcgg
      661 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tcaagtttgt
      721 acaaaaaagc aggctccgcg tttagcttat ggggagcagc aagagcaagc ccaagtctag
      781 aatgaacgac gtagccattg tgaaggaggg ctggctgcac aaacgagggg aatatattaa
      841 aacctggcgg ccacgctact tcctcctcaa gaacgatggc acctttattg gctacaagga
      901 acggcctcag gatgtggatc agcgagagtc cccactcaac aacttctcag tggcacaatg
      961 ccagctgatg aagacagagc ggccaaggcc caacaccttt atcatccgct gcctgcagtg
     1021 gaccacagtc attgaacgca ccttccatgt ggaaacgcct gaagagcggg aagaatgggc
     1081 caccgccatt caaactgtgg ccgatggact caaaaggcag gaagaaa
//
