LOCUS       sequence-12287-e       10087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, 
            Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S
  TITLE     Optimisation of bioluminescent reporters for use with mycobacteria.
  JOURNAL   PLoS One. 2010 May 24;5(5):e10777.
  PUBMED    20520722
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-05-24"; volume: "5(5)"; pages: "e10777"
COMMENT     Sequence Label: pMV306hsp+Lux
FEATURES             Location/Qualifiers
     source          1..10087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1896..1915)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1907..2722
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1980..1999)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3054..3642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3543..3562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4321..5403
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5418..6401
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             6425..7867
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7879..8802
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             8828..9940
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      10005..10051
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
      661 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
      721 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
      781 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
      841 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
      901 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
      961 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     1021 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     1081 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     1141 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     1201 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     1261 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     1321 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     1381 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     1441 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     1501 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     1561 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     1621 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     1681 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     1741 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagct agcaacaaag
     1801 cgacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1861 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1921 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1981 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2041 gaagccccat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2101 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2161 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     2221 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     2281 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     2341 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2401 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataatct
     2461 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2521 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2581 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2641 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2701 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2761 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     2821 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     2881 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     2941 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     3001 cctcactagt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3181 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3421 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3781 cgaacgaccg agcgcaacgc gtgcggccgc tctagaggtg accacaacga cgcgcccgct
     3841 ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg ttgtcgttgg cggtcatggg
     3901 ccgaacatac tcacccggat cggagggccg aggacaaggt cgaacgaggg gcatgacccg
     3961 gtgcggggct tcttgcactc ggcataggcg agtgctaaga ataacgttgg cactcgcgac
     4021 cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg tcgcagcgag tggcagcgag
     4081 gacaacttga gccgtccgtc gcgggcactg cgcccggcca gcgtaagtag cggggttgcc
     4141 gtcacccggt gacccccggt ttcatccccg atccggagga atcacttcgc aatggccaag
     4201 acaattgcgg atcccccggg ctgcaggaat tcgaatggcc atgggacgtc gacctgaggt
     4261 aattataacc cgggccctat atatggatcc aattgcaatg atcgtcgaca ggaggactct
     4321 atgaaatttg gaaacttttt gcttacatac caacctcccc aattttctca aacagaggta
     4381 atgaaacgtt tggttaaatt aggtcgcatc tctgaggagt gtggttttga taccgtatgg
     4441 ttactggagc atcatttcac ggagtttggt ttgcttggta acccttatgt cgctgctgca
     4501 tatttacttg gcgcgactaa aaaattgaat gtaggaactg ccgctattgt tcttcccaca
     4561 gcccatccag tacgccaact tgaagatgtg aatttattgg atcaaatgtc aaaaggacga
     4621 tttcggtttg gtatttgccg agggctttac aacaaggact ttcgcgtatt cggcacagat
     4681 atgaataaca gtcgcgcctt agcggaatgc tggtacgggc tgataaagaa tggcatgaca
     4741 gagggatata tggaagctga taatgaacat atcaagttcc ataaggtaaa agtaaacccc
     4801 gcggcgtata gcagaggtgg cgcaccggtt tatgtggtgg ctgaatcagc ttcgacgact
     4861 gagtgggctg ctcaatttgg cctaccgatg atattaagtt ggattataaa tactaacgaa
     4921 aagaaagcac aacttgagct ttataatgaa gtggctcaag aatatgggca cgatattcat
     4981 aatatcgacc attgcttatc atatataaca tctgtagatc atgactcaat taaagcgaaa
     5041 gagatttgcc ggaaatttct ggggcattgg tatgattctt atgtgaatgc tacgactatt
     5101 tttgatgatt cagaccaaac aagaggttat gatttcaata aagggcagtg gcgtgacttt
     5161 gtattaaaag gacataaaga tactaatcgc cgtattgatt acagttacga aatcaatccc
     5221 gtgggaacgc cgcaggaatg tattgacata attcaaaaag acattgatgc tacaggaata
     5281 tcaaatattt gttgtggatt tgaagctaat ggaacagtag acgaaattat tgcttccatg
     5341 aagctcttcc agtctgatgt catgccattt cttaaagaaa aacaacgttc gctattatat
     5401 tagctaagga gaaagaaatg aaatttggat tgttcttcct taacttcatc aattcaacaa
     5461 ctgttcaaga acaaagtata gttcgcatgc aggaaataac ggagtatgtt gataagttga
     5521 attttgaaca gattttagtg tatgaaaatc atttttcaga taatggtgtt gtcggcgctc
     5581 ctctgactgt ttctggtttt ctgctcggtt taacagagaa aattaaaatt ggttcattaa
     5641 atcacatcat tacaactcat catcctgtcg ccatagcgga ggaagcttgc ttattggatc
     5701 agttaagtga agggagattt attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc
     5761 atttttttaa tcgcccggtt gaatatcaac agcaactatt tgaagagtgt tatgaaatca
     5821 ttaacgatgc tttaacaaca ggctattgta atccagataa cgatttttat agcttcccta
     5881 aaatatctgt aaatccccat gcttatacgc caggcggacc tcggaaatat gtaacagcaa
     5941 ccagtcatca tattgttgag tgggcggcca aaaaaggtat tcctctcatc tttaagtggg
     6001 atgattctaa tgatgttaga tatgaatatg ctgaaagata taaagccgtt gcggataaat
     6061 atgacgttga cctatcagag atagaccatc agttaatgat attagttaac tataacgaag
     6121 atagtaataa agctaaacaa gagacgcgtg catttattag tgattatgtt cttgaaatgc
     6181 accctaatga aaatttcgaa aataaacttg aagaaataat tgcagaaaac gctgtcggaa
     6241 attatacgga gtgtataact gcggctaagt tggcaattga aaagtgtggt gcgaaaagtg
     6301 tattgctgtc ctttgaacca atgaatgatt tgatgagcca aaaaaatgta atcaatattg
     6361 ttgatgataa tattaagaag taccacatga aatataccta ataggtacca ggaggatggc
     6421 aaatatgact aaaaaaattt cattcattat taacggccag gttgaaatct ttcccgaaag
     6481 tgatgattta gtgcaatcca ttaattttgg tgataatagt gtttacctgc caatattgaa
     6541 tgactctcat gtaaaaaaca ttattgattg taatggaaat aacgaattac ggttgcataa
     6601 cattgtcaat tttctctata cggtagggca aagatggaaa aatgaagaat actcaagacg
     6661 caggacatac attcgtgact taaaaaaata tatgggatat tcagaagaaa tggctaagct
     6721 agaggccaat tggatatcta tgattttatg ttctaaaggc ggcctttatg atgttgtaga
     6781 aaatgaactt ggttctcgcc atatcatgga tgaatggcta cctcaggatg aaagttatgt
     6841 tcgggctttt ccgaaaggta aatctgtaca tctgttggca ggtaatgttc cattatctgg
     6901 gatcatgtct atattacgcg caattttaac taagaatcag tgtattataa aaacatcgtc
     6961 aaccgatcct tttaccgcta atgcattagc gttaagtttt attgatgtag accctaatca
     7021 tccgataacg cgctctttat ctgttatata ttggccccac caaggtgata catcactcgc
     7081 aaaagaaatt atgcgacatg cggatgttat tgtcgcttgg ggagggccag atgcgattaa
     7141 ttgggcggta gagcatgcgc catcttatgc tgatgtgatt aaatttggtt ctaaaaagag
     7201 tctttgcatt atcgataatc ctgttgattt gacgtccgca gcgacaggtg cggctcatga
     7261 tgtttgtttt tacgatcagc gagcttgttt ttctgcccaa aacatatatt acatgggaaa
     7321 tcattatgag gaatttaagt tagcgttgat agaaaaactt aatctatatg cgcatatatt
     7381 accgaatgcc aaaaaagatt ttgatgaaaa ggcggcctat tctttagttc aaaaagaaag
     7441 cttgtttgct ggattaaaag tagaggtgga tattcatcaa cgttggatga ttattgagtc
     7501 aaatgcaggt gtggaattta atcaaccact tggcagatgt gtgtaccttc atcacgtcga
     7561 taatattgag caaatattgc cttatgttca aaaaaataag acgcaaacca tatctatttt
     7621 tccttgggag tcatcattta aatatcgaga tgcgttagca ttaaaaggtg cggaaaggat
     7681 tgtagaagca ggaatgaata acatatttcg agttggtgga tctcatgacg gaatgagacc
     7741 gttgcaacga ttagtgacat atatttctca tgaaaggcca tctaactata cggctaagga
     7801 tgttgcggtt gaaatagaac agactcgatt cctggaagaa gataagttcc ttgtatttgt
     7861 cccataatag gtaaaagtat ggaaaatgaa tcaaaatata aaaccatcga ccacgttatt
     7921 tgtgttgaag gaaataaaaa aattcatgtt tgggaaacgc tgccagaaga aaacagccca
     7981 aagagaaaga atgccattat tattgcgtct ggttttgccc gcaggatgga tcattttgct
     8041 ggtctggcgg aatatttatc gcggaatgga tttcatgtga tccgctatga ttcgcttcac
     8101 cacgttggat tgagttcagg gacaattgat gaatttacaa tgtctatagg aaagcagagc
     8161 ttgttagcag tggttgattg gttaactaca cgaaaaataa ataacttcgg tatgttggct
     8221 tcaagcttat ctgcgcggat agcttatgca agcctatctg aaatcaatgc ttcgttttta
     8281 atcaccgcag tcggtgttgt taacttaaga tattctcttg aaagagcttt agggtttgat
     8341 tatctcagtc tacccattaa tgaattgccg gataatctag attttgaagg ccataaattg
     8401 ggtgctgaag tctttgcgag agattgtctt gattttggtt gggaagattt agcttctaca
     8461 attaataaca tgatgtatct tgatataccg tttattgctt ttactgcaaa taacgataat
     8521 tgggtcaagc aagatgaagt tatcacattg ttatcaaata ttcgtagtaa tcgatgcaag
     8581 atatattctt tgttaggaag ttcgcatgac ttgagtgaaa atttagtggt cctgcgcaat
     8641 ttttatcaat cggttacgaa agccgctatc gcgatggata atgatcatct ggatattgat
     8701 gttgatatta ctgaaccgtc atttgaacat ttaactattg cgacagtcaa tgaacgccga
     8761 atgagaattg agattgaaaa tcaagcaatt tctctgtctt aaaggatcct gaggaggaaa
     8821 aacaggtatg acttcatatg ttgataaaca agaaattaca gcaagctcag aaattgatga
     8881 tttgattttt tcgagcgatc cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa
     8941 gaaacttgtg cttgatgcat ttcgtaatca ttataaacat tgtcgagaat atcgtcacta
     9001 ctgtcaggca cacaaagtag atgacaatat tacggaaatt gatgacatac ctgtattccc
     9061 aacatcggtt tttaagttta ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg
     9121 gtttaccagt agcggcacga atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat
     9181 tgagagactc ttaggctctg tgagttatgg catgaaatat gttggtagtt ggtttgatca
     9241 tcaaatagaa ttagtcaatt tgggaccaga tagatttaat gctcataata tttggtttaa
     9301 atatgttatg agtttggtgg aattgttata tcctacgaca tttaccgtaa cagaagaacg
     9361 aatagatttt gttaaaacat tgaatagtct tgaacgaata aaaaatcaag ggaaagatct
     9421 ttgtcttatt ggttcgccat actttattta tttactctgc cattatatga aagataaaaa
     9481 aatctcattt tctggagata aaagccttta tatcataacc ggaggcggct ggaaaagtta
     9541 cgaaaaagaa tctctgaaac gtgatgattt caatcatctt ttatttgata ctttcaatct
     9601 cagtgatatt agtcagatcc gagatatatt taatcaagtt gaactcaaca cttgtttctt
     9661 tgaggatgaa atgcagcgta aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc
     9721 tgaaacgttg aaacctgtac ctgatggaac gccggggttg atgagttata tggatgcgtc
     9781 agcaaccagt tatccagcat ttattgttac cgatgatgtc gggataatta gcagagaata
     9841 tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa
     9901 agggtgtgct ttaagcttaa ccgaagcgtt tgatagttga tatcctgcaa tcaagcttat
     9961 cgatgtcgac gtagttaact agcgtacgat cgactgccag gcatcaaata aaacgaaagg
    10021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtccggccat catggccgcg
    10081 gtgatca
//
