LOCUS       sequence-122888-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: RBS-RFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_8_RBS34- mCherry -B0015
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      264..335
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      351..378
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(451..470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(452..470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      301 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      361 cgggtgggcc tttctgcgtt tataggctcg ggagacctat cgtatcttgc tgaaaaactc
      421 gagccatccg gaagatctgg cggccgctct ccctatagtg agtcgtatta cgccggatgg
      481 atatggtgtt caggcacaag tgttaaagca gttgatttta ttcactatga tgaaaaaaac
      541 aatgaatgga acctgctcca agttaaaaat agagataata ccgaaaactc atcgagtagt
      601 aagattagag ataatacaac aataaaaaaa tggtttagaa cttactcaca gcgtgatgct
      661 actaattggg acaattttcc agatgaagta tcatctaaga atttaaatga agaagacttc
      721 agagcttttg ttaaa
//
