LOCUS       sequence-122900-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint frame selector +1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     167..189
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     205..221
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        248..496
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     248..268
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     419..438
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        517..592
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
       61 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      121 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      181 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      241 cggatccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
      301 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
      361 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      421 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      481 gaaaggacga aacaccggcc agtacccaaa aagcgggttt tagagctaga aatagcaagt
      541 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
      601 gaattccgat gtacgggcca gatatacgcg ttgacattga ttattgtcta gttattaata
      661 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      721 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      781 gacgtatgtt cccatagtaa
//
