LOCUS       sequence-122907-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of alpha-bungarotoxin mRNA that can be 
            injected into embryos..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Swinburne IA, Mosaliganti KR, Green AA, Megason SG
  TITLE     Improved Long-Term Imaging of Embryos with Genetically Encoded 
            alpha-Bungarotoxin.
  JOURNAL   PLoS One. 2015 Aug 5;10(8):e0134005. doi: 
            10.1371/journal.pone.0134005. eCollection 2015.
  PUBMED    26244658
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-5"; volume: "10(8):e0134005. doi"; pages: " 
            10.1371/journal.pone.0134005. eCollection 2015"
COMMENT     Sequence Label: pmtb-t7-alpha-bungarotoxin
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    405..539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(429..448)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     483..502
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(631..768,769..780)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 atttaggtga cactatagaa gagtactaat acgactcact atagggagaa gctacttgtt
       61 ctttttgcag gatccgccac catgaagacc ctgctgctga ccctggtggt ggtgacaatc
      121 gtgtgcctgg acctgggata caccatcgtg tgccacacca ccgccaccag ccctatcagc
      181 gctgtgacct gccctcctgg cgagaacctg tgctacagaa agatgtggtg cgacgccttc
      241 tgtagcagca gaggcaaggt ggtcgagctg ggatgcgccg ctacctgccc tagcaagaaa
      301 ccttacgaag aggtgacctg ctgtagcacc gacaagtgca accctcaccc taagcagcgc
      361 cctggatgat ctgcagtaag aattcatacg tatccggaac cggtgatcca gacatgataa
      421 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      481 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      541 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
      601 gatatcccgg gtttaaacgc atgcagatct aatactcaag tacaatttta atggagtact
      661 tttttacttt tactcaagta agattctagc cagatacttt tacttttaat tgagtaaaat
      721 tttccctaag tacttgtact ttcacttgag taaaattttt gagtactttt tacacctctg
//
