LOCUS       sequence-122912-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing mKusabira-Orange2 for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO mKO2
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    345..366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(381..386,387..404,405..411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     424..446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     443..459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     443..459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        477..495
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     477..494
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      301 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      361 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      421 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
      481 aggtgacact atagaatact caagctatgc atcaagcttg gtaccgagct cggatcctaa
      541 ctcgaacgct agctgtgcga tcgtttacca tggtgtccgt gatcaagccc gagatgaaga
      601 tgcgttacta catggacggc agcgtgaacg gccacgagtt caccatcgag ggcgagggca
      661 caggcagacc ttacgagggc caccaggaaa tgaccctgag agtgaccatg gccgagggcg
      721 gacctatgcc cttcgccttc gatctggtgt cccacgtgtt ctgctacggc cacagagtgt
      781 tcaccaagta ccccgaggaa atccccgact acttcaagca ggccttcccc gagggcctga
      841 gctgggagag aagcctggaa ttcgaggacg gcggcagcgc ctctgtgtct gcccacatct
      901 ctctgagagg caacaccttc taccacaaga gcaagttcac cggcgtgaac ttccccgccg
      961 acggccccat catgcagaac cagtctgtgg actgggagcc cagcaccgag aagatcacag
     1021 cctctgacgg cgtgctgaag ggcgacgtga ccatgtacct gaagctggaa ggcggcggaa
     1081 accacaagtg ccagatgaag accacctaca
//
