LOCUS       sequence-122913-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing mKusabira-Orange2 for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO mKO2
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     232..254
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     246..263
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     247..263
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     270..289
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        270..288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggccagggg gatcaccatc cgtcgccccg gcgtgtcaat aatatcactc tgtacatcca
       61 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc cgtacgtata
      121 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
      181 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
      241 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc
      301 ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat tccagcacac
      361 tggcggccgt tactagtgga tccagagtaa ttcaacccca aacaacaacg tttgctgtgg
      421 gccacggcat cttccacctg ttcggtgatg ttgccctcgg tcttgcgcac gagcctgtgg
      481 ccgatgtagt ggtcgccggg catttccagg atttctttgg cggccttgta ggtggtcttc
      541 atctggcact tgtggtttcc gccgccttcc agcttcaggt acatggtcac gtcgcccttc
      601 agcacgccgt cagaggctgt gatcttctcg gtgctgggct cccagtccac agactggttc
      661 tgcatgatgg ggccgtcggc ggggaagttc acgccggtga acttgctctt gtggtagaag
      721 gtgttgcctc tcagagagat gtgggcagac acagaggcgc tgccgccgtc ctcgaattcc
      781 aggcttctct cccagctcag gccctcgggg aaggcctgct tgaagtagtc ggggatttcc
      841 tcggggtact tggtgaacac tctgtggccg tagcagaaca cgtgggacac cagatcgaag
      901 gcgaagggca taggtccgcc ctcggccatg gtcactctca gggtcatttc ctggtgg
//
