LOCUS       sequence-122920-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Nesprin3-C-18
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..594
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 acaatggcta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggacaaagac
       61 tgcgaaatga agcgcaccac cctggatagc cctctgggca agctggaact gtctgggtgc
      121 gaacagggcc tgcaccgtat catcttcctg ggcaaaggaa catctgccgc cgacgccgtg
      181 gaagtgcctg ccccagccgc cgtgctgggc ggaccagagc cactgatgca ggccaccgcc
      241 tggctcaacg cctactttca ccagcctgag gccatcgagg agttccctgt gccagccctg
      301 caccacccag tgttccagca ggagagcttt acccgccagg tgctgtggaa actgctgaaa
      361 gtggtgaagt tcggagaggt catcagctac agccacctgg ccgccctggc cggcaatccc
      421 gccgccaccg ccgccgtgaa aaccgccctg agcggaaatc ccgtgcccat tctgatcccc
      481 tgccaccggg tggtgcaggg cgacctggac gtggggggct acgagggcgg gctcgccgtg
      541 aaagagtggc tgctggccca cgagggccac agactgggca agcctgggct gggttccgga
      601 ctcagatctc gagagtccag tggatattcg tctcggtcgg gtggaagcac tcagcagccc
      661 caggacgact ttgacaggag cgtggaggat gcccaggcat ggatgaaggc tgtgcaggac
      721 cagctgcagg tcaatgacaa cacgcaggga ccccgcgcgg ccctggaggc caggctgtgg
      781 gagaccgaga aaatatgcca gctggagccc gaggggcgtg tgagggtgga cctcgtgcta
      841 cggatggctg aagccctctt ggcatgctgc cctggggacc agaagcccgg gatcctggcc
      901 cggctgaagg acatcaaggc ccaatgggag gagacagtca cctacatgac tcactgtcac
      961 agccgcatcg agtgggtgtg gctgcactgg agcgagtacc tgctggcccg agatgagttc
     1021 taccgctggg ttccagaaga tgatggtcac actggagccc ccacatcgag ctcagctggt
     1081 ctgaggagag cagtgcagct gaccacgccc agtgctgctg cacacgtgaa acacagccgt
     1141 gctctgacgc tgctggagag cagcttctat caaggatcgg accagctgac gaatgccgag
     1201 gaattgaagg cgtggatatc caagtgtca
//
