LOCUS       sequence-122924-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T2-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..778
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTETLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tggtttagtg aaccgtcaga atccgctagc gctaccggtc gccaccatgg tgtctaaggg
       61 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
      121 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
      181 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
      241 cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg atttctttaa
      301 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
      361 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
      421 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
      481 ggccaacacc gagaccctgt accccgctga cggcggcctg gaaggcagaa ccgacatggc
      541 cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa
      601 gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag
      661 aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata
      721 ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc tgtacaagtc
      781 cggactcaga tctcgagctc aagcttcgat ggcgactgcg acccccgtgc cgccgcggat
      841 gggcagccgc gctggcggcc ccaccacgcc gctgagcccc
//
