LOCUS       sequence-122950-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: 490 / 587, Emission: 511 /
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PS-CFP2-mCherry-FRET-10
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..763
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     regulatory      788..797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tgagcaaggg
       61 cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg
      121 ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct
      181 gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tggccaccct
      241 gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt
      301 caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg
      361 caactacaag acgcgcgccg aggtgaagtt cgagggcgat accctggtga gtcgcatcga
      421 gctgaccggc actgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa
      481 ctacaacgcc accaatgtgt acatcgtggc cgacaaggcc aggaatggca tcaaggtgaa
      541 cttcaaggtc cgccacaaca tcaaggatgg cagcgtgcag ctggccgacc actaccagca
      601 gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca
      661 gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctacc tcgagttcgt
      721 gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtccggac tcagatcccc
      781 accggtcgcc accatggtga
//
