LOCUS       sequence-122956-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lenti sgRNA cloning backbone with MS2 loops at 
            tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains 
            BsmBI sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti sgRNA(MS2)_zeo backbone
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        95..343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     95..115
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     266..285
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        381..399
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        451..469
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        612..823
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
       61 gacagcagag atccagtttg gttaattagc tagcgagggc ctatttccca tgattccttc
      121 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
      181 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
      241 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
      301 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggagacg ggataccgtc
      361 tctgttttag agctaggcca acatgaggat cacccatgtc tgcagggcct agcaagttaa
      421 aataaggcta gtccgttatc aacttggcca acatgaggat cacccatgtc tgcagggcca
      481 agtggcaccg agtcggtgct ttttttggat cctgcaaaga tggataaagt tttaaacaga
      541 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      601 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      661 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      721 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      781 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      841 ttcccgcggg cctggcctct ttacgg
//
