LOCUS       sequence-12296-e        2691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2691)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72031
FEATURES             Location/Qualifiers
     source          1..2691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..38
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     primer_bind     complement(71..88)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(150..174)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(324..343)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             335..1150
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             335..1150
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(408..427)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             1360..2019
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1426..1445)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2040..2134
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2170..2526
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    2588..2612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatctcagcc ggagctcgca ccagaagacc cggaagactg ctaaggatcc taactcgctc
       61 ctcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      121 cagctcactc aaaggcggta atcaattcga cccagctttc ttgtacaaag tggttgatcg
      181 gatctgctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc
      241 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
      301 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
      361 ttgctccagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
      421 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
      481 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
      541 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
      601 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
      661 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
      721 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
      781 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
      841 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
      901 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
      961 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     1021 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     1081 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     1141 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     1201 acgggaggat ctctgaagac tctcgagctg caggactcac agctcctcgg atctttgatc
     1261 gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     1321 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     1381 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     1441 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     1501 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     1561 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
     1621 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     1681 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     1741 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     1801 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     1861 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     1921 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1981 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg
     2041 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     2101 cggttgccgc cgggcgtttt ttattggtga gaatccaagc ctcggatcta gcagttcaac
     2161 ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat gtttaacgta
     2221 ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc tctcatggct
     2281 aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa taaaattaat
     2341 ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa aaaagaatat
     2401 ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag ggatgtaacg
     2461 cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa cacttaacgg
     2521 ctgacatggg aattagccat gggcccgtgc gaatcacgga tctcccgatc aagaattgcc
     2581 attatcaaca agtttgtaca aaaaagcagg ctccgaattg gtatcacgag gcagaatttc
     2641 agataaaaaa aatccttagc tttcgctaag gatctgaagt ggaattcatg a
//
