LOCUS       sequence-122965-         142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 N863A nickase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 142)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX429 SpCas9 N863A nickase
FEATURES             Location/Qualifiers
     source          1..142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
       61 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      121 tcctgcaggc agctgcgcgc tc
//
