LOCUS       sequence-122970-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and 
            attB donor plasmid containing GFP is present in the cell, 
            recombination of the donor into the reporter leads to expression of 
            GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: Bxb1-surrogate-reporter
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    116..163
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    307..428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(344..363)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     398..417
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(435..890)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(572..593)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     784..803
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tcatgggttt
      121 gtctggtcaa ccaccgcggt ctcagtggtg tacggtacaa acctaactaa ctaagtacaa
      181 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
      241 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      301 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      421 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      481 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      541 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      601 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      661 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      721 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      781 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      841 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
      901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aa
//
