LOCUS       sequence-122971-        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and 
            attB donor plasmid containing GFP is present in the cell, 
            recombination of the donor into the reporter leads to expression of 
            GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: Bxb1-surrogate-reporter
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    338..385
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     410..429
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(728..747)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
      181 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
      241 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
      301 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
      361 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
      421 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
      481 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
      541 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
      601 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
      661 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
      721 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
      781 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
      841 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
      901 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
      961 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     1021 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     1081 cggcaggagc aaggtgagat gacaggagat cctgccc
//
