LOCUS       sequence-122973-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for cotranslational expression of tagRFP-Bxb1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: pCAG-tagRFP-NLS-HA-Bxb1
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    58..282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     271..288
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cctcgaggct agaactagtg gatctcgagc cccagctggt tctttccgcc tcagaagcca
       61 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
      121 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
      181 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
      241 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcgag
      301 ctctgaccta ggacatccca gtgtgcagtc tttcgaccac ggaccccagt ctcaggtgct
      361 gttcatactc ctgcaggtcg ccaaaatcga tggttcgagt caggccaccg cgcacgtcga
      421 atgtgagccg gacattcatg gatctcagcc atgtgttctt ggctgcggtg tcctgctccc
      481 tccaccaatc cccaaacctc tgtccggttt ctcgccactc ccatccagag ggcctggcct
      541 ccaggccctc cagttcctcc tgtctagcag cgagtgcagc aattcgtgcg tccagagctt
      601 ctctctgtgg ggacccagct ctatatgcag gagatccgat gagggaagtc agatccacga
      661 gctcggcatt gacttctgcc agctccacag cggagtcaga tcctgccacc cacacttttt
      721 ccagccgctc agcgtcaccc aggagatcca gcacctgttc ctcgcaaaag gcgtcccact
      781 cagccatggc cactgtcccg tttccacagt gcttagggaa ccccatgctc ctgcatcgat
      841 agcggggatg tttgcgccct ccgccggcaa acttgtatgc tggctctcca cagacggcgc
      901 agaacagcac cctcaggagc aggcttgggg tgctcacggc aggtttggcg cgactagtct
      961 tgaccagttc tgcccgcaga gcttcgagct gctctctggt gagaataggc tcagccctca
     1021 ccagaggggc tccgtcatcg tcccgga
//
