LOCUS       sequence-122987-        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into
            a MIN-tagged locus using Neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-mCh-Dnmt3b6-Poly(A)-NeoR
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..162
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    333..380
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     405..424
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(723..742)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
       61 tgcaccttga agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc
      121 acggatgatc ctgacgacgg agaccgccgt cgtcgacaag ccgggatctc gatgtaagct
      181 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
      241 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
      301 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
      361 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
      421 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
      481 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
      541 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
      601 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
      661 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
      721 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
      781 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
      841 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
      901 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
      961 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     1021 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     1081 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
//
