LOCUS       sequence-122990-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive selectable expression of C-term V5 tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   McHugh CA, Chen CK, Chow A, Surka CF, Tran C, McDonel P, 
            Pandya-Jones A, Blanco M, Burghard C, Moradian A, Sweredoski MJ, 
            Shishkin AA, Su J, Lander ES, Hess S, Plath K, Guttman M
  TITLE     The Xist lncRNA interacts directly with SHARP to silence 
            transcription through HDAC3.
  JOURNAL   Nature. 2015 May 14;521(7551):232-6. doi: 10.1038/nature14443. Epub 
            2015 Apr 27.
  PUBMED    25915022
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14443"; journalName: "Nature"; date: "2015-05-14- 
            14"; volume: "521"; issue: "7551"; pages: "232-6"
COMMENT     Sequence Label: pCAG-GW-V5-Hygro
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(102..108,109..126,127..132))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(157..281)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(310..329)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(391..413)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc
      121 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
      181 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      241 tcattttacg tttctcgttc agcttttttg tacaaacttg tggaattctt tgccaaaatg
      301 atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg
      361 aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga accccgccct
      421 gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgaggct ggagagggag
      481 aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct tcctgccggc
      541 gccgcaccgc ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc cagatttcgg
      601 ctccgcacag atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt accataaaag
      661 gcaatggctg cggctcgccg cgcctcgaca gccgccggcg ctccggggg
//
