LOCUS       sequence-123006-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9nlsHis6 Bacterial Expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Fu BX, Hansen LL, Artiles KL, Nonet ML, Fire AZ
  TITLE     Landscape of target:guide homology effects on Cas9-mediated 
            cleavage.
  JOURNAL   Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 
            10.1093/nar/gku1102. Epub 2014 Nov 15.
  PUBMED    25399416
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1102"; journalName: "Nucleic Acids Res"; date: 
            "2014-12-16- 16"; volume: "42"; issue: "22"; pages: "13778-87"
COMMENT     Sequence Label: pHO4d-Cas9
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(53..70)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..121)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tagcagccgg atcctagttc agatcttctt cgctgatcag tttctgttcg ccgtgatggt
       61 ggtgatggtg gcccgaattt cctgcagctc caccgctcga gactttcctc ttcttcttgg
      121 gagaaccgtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg
      181 attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa
      241 ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac gtaaataaat
      301 gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta tatgcactaa
      361 gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca ctgatttgct
      421 caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt tgttcgttat
      481 cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa ttcacatatt
      541 tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc agcatccgtt
      601 tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt aagtcttttt
      661 taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca aaggaacttc
      721 tttccataat tgtgatccct agtaactctt taacggattt taacttcttc gatttccc
//
