LOCUS       sequence-123007-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral plasmid for establishment of blasticidin resistance 
            Cre-reporter cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: FFiBlast MSCV Puro
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..203
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     219..235
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     265..281
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     266..282
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(315..348)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(395..428)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(395..428)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(435..833)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      830..839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    865..898
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    913..946
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    913..946
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(948..964)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(948..964)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1040..1063)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
       61 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
      121 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
      181 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
      241 ctctaggcgc cggaattaga tctctcgagg tcgacggtat cgataagctt gatatcgaat
      301 tcctgcagcc cgggataact tcgtatagta cacattatac gaagttatgg ctaagctttg
      361 tgccatgcca ttgatgctag cacagcctga atggataact tcgtatagga taccttatac
      421 gaagttatac cggtttagcc ctcccacaca taaccagagg gcagcaattc acgaatccca
      481 actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg atgcagatcg
      541 agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc atttccgatc
      601 gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag caccacgagt
      661 tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat gcggccgtcg
      721 ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct gttgattgta
      781 gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc catggtggcg
      841 cgccagtaac atgtagtagt aaacataact tcgtataatg tgtactatac gaagttatgg
      901 cttctagctg agataacttc gtataaggta tcctatacga agttatggat ccactagttc
      961 tagagcggcc gctcgagtct agatcgaggt taacgaattc taccgggtag gggaggcgct
     1021 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     1081 aagtggcctc tggcctcgca cacattccac atc
//
