LOCUS       sequence-123021-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus for establishment of doxycycline-inducible cell line 
            expression CloverCP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pTRE:CloverCP-EFS:ZeoR
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..483)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(528..739)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(806..925)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(929..976)
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
ORIGIN
        1 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg
       61 ggcgcgccgc ggtctagaag agtaattcaa ccccaaacaa caacgttttc agtcctgctc
      121 ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca
      181 cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac
      241 gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt
      301 gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac
      361 cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa
      421 ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc
      481 catccgctta agctgtagcg ctaatgtcgg gtcccgcgtc acgacacctg tgttctggcg
      541 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
      601 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
      661 caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc ggggactgtg
      721 ggcgatgtgc gctctgccca ctgacgggca ccggagccac tcgaaggcct cgggattcct
      781 aggaacagcg gtttgcggcc gcttagacgt tgatcctggc gctggcgcaa gcagcagggt
      841 gtctatccat gccgctctcc tgggcgcagc tcatgggcag ggtgccggcg gcctgctcct
      901 ccacctcggg agggaagccg tggctgttaa ggtggatcac aaagtggctt aagctactga
      961 accagttctt gcaagcagaa ttgcatttag g
//
