LOCUS       sequence-123027-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing CMV for generation of GMAP-compatible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO CMV
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    345..366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(381..386,387..404,405..411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     424..446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     443..459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     443..459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        477..495
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     477..494
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        603..982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      301 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      361 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      421 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
      481 aggtgacact atagaatact caagctatgc atcaagcttg gtaccgagct cggatcagtg
      541 tgagggagtg taaagctggt tttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      601 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      661 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      721 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      781 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      841 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      901 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      961 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1021 gcggtttgac tcacggggat ttccaagtcc
//
