LOCUS       sequence-123028-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing CMV for generation of GMAP-compatible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO CMV
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     244..266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     258..275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     259..275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     282..301
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(470..673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(499..519)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        674..977
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcagacgtgc actggccagg gggatcacca tccgtcgccc cggcgtgtca ataatatcac
       61 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      121 gccgtacgta taggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      181 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      241 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      301 gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
      361 attccagcac actggcggcc gttactagtg gatcccgctt aagctgtagc gctaatgtcg
      421 ggtcaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      481 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      541 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      601 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
      661 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
      721 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
      781 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
      841 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
      901 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
      961 ccgtaagtta tgtaacgcgg aactcc
//
