LOCUS       sequence-123031-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing Cre-2A-GFP for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO Cre-2A-GFP
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctcggatc ctaactcgaa cgctagctgt gcgatcgttt accatgccca agaagaagag
       61 gaaggtgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac
      121 gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga
      181 gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa
      241 taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca
      301 ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca
      361 tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg
      421 gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg
      481 cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg
      541 taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag
      601 gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag
      661 aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa
      721 actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt
      781 ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg
      841 cgccctggaa gggatttttg aagcaactca ccgattgatt tacggcgcta aggatgactc
      901 tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat
      961 ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa
     1021 tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct
     1081 g
//
