LOCUS       sequence-123039-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing Venus for generation of GMAP-compatible 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO Venus
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     244..266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     258..275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     259..275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     282..301
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(429..680)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(471..492)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     907..926
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagacgtgc actggccagg gggatcacca tccgtcgccc cggcgtgtca ataatatcac
       61 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      121 gccgtacgta taggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      181 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      241 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      301 gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
      361 attccagcac actggcggcc gttactagtg gatccagagt aattcaaccc caaacaacaa
      421 cgtttttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      481 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtagctc
      541 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      601 tggtcggcga gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttcgccttg
      661 atgccgttct tctgcttgtc ggccactgag attcgacaga tagacaggtc tgcggctgcg
      721 tggttcataa ctttccgttt caggtcgttg gggattttgc aggcgggcct cgtgatatag
      781 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      841 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      901 gcgcgggtct tgtagttgcc gtcgtctttg aagaagatgg tg
//
