LOCUS       sequence-123047-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Liu F, Wagner S, Campbell RB, Nickerson JA, Schiffer CA, Ross AH
  TITLE     PTEN enters the nucleus by diffusion.
  JOURNAL   J Cell Biochem. 2005 Oct 1. 96(2):221-34.
  PUBMED    16088943
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem. 2005 Oct 1. 96(2):221-34."
COMMENT     Sequence Label: pcDNA3 GFP GFP
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(62..775,776..778,779..781))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     456..475
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     715..736
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcgagcggcc gccactgtgc tggatatctg cagaattcca ccacactgga ctagtggatc
       61 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      121 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      181 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      241 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      301 cgttcttctg cttgtcggcc gtgatataga cgttgtggct gttgtagttg tactccagct
      361 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      421 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      481 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      541 gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc gtagccgaag gtggtcacga
      601 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      661 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      721 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
      781 tggcagtcta gagtcgacct gcaggcatgc aagcttcttg tacagctcgt ccatgccgag
      841 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      901 g
//
