LOCUS       sequence-123048-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-mCherry-P2A-strepII-PPPCB
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             265..321
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(331..354,355..390,391..414)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
       61 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      241 ctgtacaagg gatccggaag cggagctact aacttcagcc tgctgaagca ggctggagac
      301 gtggaggaga accctggacc tcggtccgaa tggagtcatc cacaatttga gaagggagga
      361 ggcagtggag gaggcagtgg aggaagtgcc tggagccacc cgcagttcga aaaaggcagt
      421 ggtggtggta gtggtggagg atgtactagt gacgacaagg cgttcaccaa ggagctggac
      481 cagtgggtcg agcagctgaa cgagtgtaag cagctgaacg agaaccaagt gcggacgctg
      541 tgcgagaagg caaaggaaat tttaacaaaa gaatcaaatg tgcaagaggt tcgttgccct
      601 gttactgtct gtggagatgt gcatggtcaa tttcatgatc ttatggaact ctttagaatt
      661 ggtggaaaat caccggatac aaactactta ttcatgggtg actatgtaga cagaggatat
      721 tattcagtgg agactgtgac tcttcttgta gcattaaagg tgcgttatcc agaacgcatt
      781 acaatatt
//
