LOCUS       sequence-123049-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-mCherry-P2A-strepII-PPPCB
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             149..160
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      507..516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc atgcctgcag
       61 gtcgactcta gagtcgcggc cgcttatagg aagtagtctg gggtgcgccg tgtaacatga
      121 ggctcaccac gacgaggcgc tgggtcaaat tgaaggaagg aatattttaa agtgtcatct
      181 aattccatga tagcagcctg gttcccacaa cgataacagt aattgggtgc actgaaaatg
      241 gtaaccacat tccgatcatg acaccaattg tatccctcca ttacaagctg gtgggcacga
      301 gaaaccagtg tgagaccatt ggcatggtta aaggtttcag aaatgtcttg tccaaatgtg
      361 tagccagcac cacgtggtga aataccccat ccaccacgat catctggatc tgaccataac
      421 agatcacaca ttgggccctc atgtggaact tcctgtaaac gatccagggc tcttatatga
      481 tccagtgtgt ctatggatgg agagaggcca ccatggaggc agaatatctg tccatctact
      541 aaagctgtaa gtggaagata atcaaagaga tctgtaaaat atttccaaac gttggcattc
      601 ccatactttc gcagacattc atcataaaag ccatatactt gggtaatttg tcggctttcg
      661 tgatttcctc tcaatattgt aatgcgttct ggataacgca cctttaatgc tacaagaaga
      721 gtcacagtct ccactgaata atatcctctg tctacatagt cacccatgaa taagtagttt
      781 gtatccggtg attttccacc aattctaaag agttccataa gatcatgaaa ttgaccatgc
      841 acatctccac agacagtaac agggcaacga acctcttgca catttgattc ttttgttaaa
      901 attt
//
