LOCUS       sequence-123050-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of mCREB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Berger AK, Green T, Siegel SJ, Nestler EJ, Hammer RP Jr
  TITLE     cAMP response element binding protein phosphorylation in nucleus 
            accumbens underlies sustained recovery of sensorimotor gating 
            following repeated D(2)-like receptor agonist treatment in rats.
  JOURNAL   Biol Psychiatry. 2011 Feb 1;69(3):288-94. doi: 
            10.1016/j.biopsych.2010.08.032. Epub 2010 Oct 30.
  PUBMED    21035786
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2010.08.032"; journalName: "Biol Psychiatry"; 
            date: "2011-02-1- 1"; volume: "69"; issue: "3"; pages: "288-94"
COMMENT     Sequence Label: AAV-mCREB
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..713
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             3..713
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(42..63)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(303..322)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     650..671
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
       61 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      121 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      181 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      241 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      301 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      361 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      421 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      481 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      541 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      601 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      661 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggtggcg
      721 ggcccatgga ttccggagca gacaaccagc agagtggaga tgctgctgta acagaagctg
      781 aaagtcaaca aatgacagtt caagcccagc cacagattgc cacattagcc caggta
//
