LOCUS       sequence-123053-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Aurora A-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR_dSV40-Aurora A-GFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..221
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      80..215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     142..161
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      235..244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgaattcag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
       61 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      181 attccagaag tagtgaggag gcttttttgg aggcctaggc tacgcgtcag tcaggccacc
      241 atggaccgat ctaaagaaaa ctgcatttca ggacctgtta aggctacagc tccagttgga
      301 ggtccaaaac gtgttctcgt gattcagcaa tttccttgtc agaatccatt acctgtaaat
      361 agtggccagg ctcagcgggt cttgcgtcct tcaaattctt cccagcgcgt tcctttgcaa
      421 gcacaaaagc ttgtctccag tcacaagccg gttcagaatc agaagcagaa gcaattgcag
      481 gcaaccagtg tacctcatcc tgtctccagg ccactgaata acacccaaaa gagcaagcag
      541 cccttgccat cggcacctga aaataatcct gaggaggaac tggcatcaaa acagaaaaat
      601 gaagaatcaa aaaagaggca gtgggctttg gaagactttg aaattggtcg ccctctgggt
      661 aaaggaaagt ttggtaatgt ttatttggca agagaaaagc aaagcaagtt tattctggct
      721 ctacgcgtgt tatttaaagc tcagctggag aaagccggag tggagcatca gctcagaaga
      781 gaagtagaaa tacagtccca ccttcggcat cctaatattc ttagactgta tggttatttc
      841 catgatgcta ccagagtcta cctaattctg gaatatgcac cacttggaa
//
