LOCUS       sequence-123054-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry-Emi1 with control UTRs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-mCherry-Emi1_control UTRs
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(296..314)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     564..583
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
       61 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggcc aatcaattgg
      121 gactcacgta cgacgctcac gcgtgccacc atggtgagca agggcgagga ggataacatg
      181 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      241 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      301 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      361 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      421 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      481 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      541 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      601 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
      661 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
      721 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
      781 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc c
//
