LOCUS       sequence-123061-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses H2B-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR_dSV40-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(37..414)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(427..637)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      433..568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(487..506)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tggatcctta gcgctggtgt acttggtgat
       61 ggccttagta ccctcggaca cggcgtgctt ggccaactcc ccaggcagca gcaggcgcac
      121 ggccgtctgg atctccctgg aggtgatggt cgagcgcttg ttgtaatgcg ccaggcggga
      181 agcctcacct gcgatgcgct cgaaaatgtc gttcacaaac gaattcatga tgcccatggc
      241 cttggacgaa atgccggtgt cagggtggac ctgcttcaga accttgtaca catagatgga
      301 atagctctcc ttgcggctgc gcttgcgctt cttgccgcct ttcttctgcg ccttagtcac
      361 cgccttcttg gagccctttt tcggggcggg agcagacttc gctggctctg gcatggtggc
      421 acgcgtagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag
      481 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
      541 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
      601 actaattgag atgcatgctt tgcatacttc tgcctgcctg aattcgatat caagcttatc
      661 gatccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
      721 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
      781 tgcactgta
//
