LOCUS       sequence-123063-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational reporter - control UTRs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ c...
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             171..647
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     primer_bind     complement(681..709)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
       61 ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggccaatca
      121 attggagaca gtcacgccat ctgctactat gaagtaggcg tacggccacc atgatcagtc
      181 tgattgcggc gttagcggta gattacgtta tcggcatgga aaacgccatg ccgtggaacc
      241 tgcctgccga tctcgcctgg tttaaacgca acaccttaaa taaacccgtg attatgggcc
      301 gccatacctg ggaatcaatc ggtcgtccgt tgccaggacg caaaaatatt atcctcagca
      361 gtcaaccgag tacggacgat cgcgtaacgt gggtgaagtc ggtggatgaa gccatcgcgg
      421 cgtgtggtga cgtaccagaa atcatggtga ttggcggcgg tcgcgttatt gaacagttct
      481 tgccaaaagc gcaaaaactg tatctgacgc atatcgacgc agaagtggaa ggcgacaccc
      541 atttcccgga ttacgagccg gatgactggg aatcggtatt cagcgaattc cacgatgctg
      601 atgcgcagaa ctctcacagc tattgctttg agattctgga gcggcgagca tgcagcaaag
      661 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      721 ggcacaaa
//
