LOCUS       sequence-123064-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational reporter - control UTRs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ c...
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..168)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(184..888)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(459..478)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     728..746
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aaattttgta atccagaggt tgattatcga taagcttgat atcaagcttg catgcctgca
       61 ggtcgactct agagtcgcgg ccgcttaagt tccatatgga tttgcggacc gaggtccagg
      121 gttctcctcc acgtctccag cctgcttcag caggctgaag ttagtagctc cgcttccgga
      181 tcccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      241 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      301 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
      361 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
      421 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
      481 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
      541 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
      601 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
      661 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
      721 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
      781 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
      841 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacac ta
//
