LOCUS       sequence-123070-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational reporter - Lox 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ L...
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..167)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(458..477)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     727..745
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc atgcctgcag
       61 gtcgactcta gagtcgcggc cgcttaagtt ccatatggat ttgcggaccg aggtccaggg
      121 ttctcctcca cgtctccagc ctgcttcagc aggctgaagt tagtagctcc gcttccggat
      181 cccttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt
      241 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag
      301 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag
      361 tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg
      421 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      481 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg
      541 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
      601 ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg
      661 taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg
      721 ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
      781 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac c
//
