LOCUS       sequence-123071-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses full length IDAX..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li
            H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A
  TITLE     Modulation of TET2 expression and 5-methylcytosine oxidation by the 
            CXXC domain protein IDAX.
  JOURNAL   Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 
            2013 Apr 7.
  PUBMED    23563267
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12052"; journalName: "Nature"; date: "2013-05-2- 2"; 
            volume: "497"; issue: "7447"; pages: "122-6"
COMMENT     Sequence Label: Myc-Idax WT
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             121..150
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             817..858
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             868..885
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attagcttgg tactaatacg actcactata gggagaccca agctggctag gtaagcttgg
       61 taccgagctc ggatccacta gtccagtgtg gtggaattcg cccttggatc cgccaccatg
      121 gagcagaaac tcatctctga agaggatctg agccaccacc ggaacgactc ccagcggctg
      181 gggaaggctg gctgtccgcc agagccgtcg ttgcaaatgg caaatactaa tttcctctcc
      241 accttatccc ccgaacactg cagacctttg gcgggggaat gcatgaacaa gctcaaatgc
      301 ggcgctgctg aagcagagat aatgaatctc cccgagcggg tggggacttt ttccgctatc
      361 ccggctttag ggggcatctc attacctcca ggggtcatcg tcatgacagc ccttcactcc
      421 cccgcagcag cctcagcagc cgtcacagac agtgcgtttc aaattgccaa tctggcagac
      481 tgcccgcaga atcattcctc ctcctcctcg tcctcctcgg ggggagctgg cggagccaac
      541 ccggccaaga agaagaggaa aaggtgtggg gtctgcgtgc cctgcaagag gctcatcaac
      601 tgtggcgtct gcagcagttg caggaaccgc aaaacgggac accagatctg caaatttaga
      661 aaatgtgaag agctaaagaa aaaacctggc acttcgctag agagaacacc tgttcccagc
      721 gctgaagcgt tccgatggtt cttttaagtt aacaagggcg aattctgcag atatccagca
      781 cagtggcggc cgctcgagtc tagagggccc ttcgaaggta agcctatccc taaccctctc
      841 ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt ttaaaccc
//
