LOCUS       sequence-123072-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses full length IDAX..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li
            H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A
  TITLE     Modulation of TET2 expression and 5-methylcytosine oxidation by the 
            CXXC domain protein IDAX.
  JOURNAL   Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 
            2013 Apr 7.
  PUBMED    23563267
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12052"; journalName: "Nature"; date: "2013-05-2- 2"; 
            volume: "497"; issue: "7447"; pages: "122-6"
COMMENT     Sequence Label: Myc-Idax WT
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(736..765)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(853..872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(854..872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc ctctagactc gagcggccgc cactgtgctg gatatctgca
      121 gaattcgccc ttgttaactt aaaagaacca tcggaacgct tcagcgctgg gaacaggtgt
      181 tctctctagc gaagtgccag gttttttctt tagctcttca cattttctaa atttgcagat
      241 ctggtgtccc gttttgcggt tcctgcaact gctgcagacg ccacagttga tgagcctctt
      301 gcagggcacg cagaccccac accttttcct cttcttcttg gccgggttgg ctccgccagc
      361 tccccccgag gaggacgagg aggaggagga atgattctgc gggcagtctg ccagattggc
      421 aatttgaaac gcactgtctg tgacggctgc tgaggctgct gcgggggagt gaagggctgt
      481 catgacgatg acccctggag gtaatgagat gccccctaaa gccgggatag cggaaaaagt
      541 ccccacccgc tcggggagat tcattatctc tgcttcagca gcgccgcatt tgagcttgtt
      601 catgcattcc cccgccaaag gtctgcagtg ttcgggggat aaggtggaga ggaaattagt
      661 atttgccatt tgcaacgacg gctctggcgg acagccagcc ttccccagcc gctgggagtc
      721 gttccggtgg tggctcagat cctcttcaga gatgagtttc tgctccatgg tggcggatcc
      781 aagggcgaat tccaccacac tggactagtg gatccgagct cggtaccaag cttacctagc
      841 cagcttgggt ctccctatag tgagtcgtat tagtaccaag ctaattcctc acgacacctg
      901 aaatggaaga aaaaa
//
