LOCUS       sequence-123089-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inverted and floxed blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: FFiBlast p5
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(105..152)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     284..303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    436..660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     649..666
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     711..727
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     711..727
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(729..762)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(729..762)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(777..810)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      836..845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
       61 acttctcgac agacgtcgcg gtgagttcag gctttttggc caaggaagtt cctatacttt
      121 ctagagaata ggaacttcgg aataggaact tctaggtacg tgaaccatca ccctaatcaa
      181 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      241 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      301 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
      361 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
      421 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
      481 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
      541 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
      601 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
      661 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagcggccgc tctagaacta
      721 gtggatccat aacttcgtat aggatacctt atacgaagtt atctcagcta gaagccataa
      781 cttcgtatag tacacattat acgaagttat gtttactact acatgttact ggcgcgccac
      841 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
      901 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
      961 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     1021 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     1081 aaatgagaac aggggcatct tg
//
