LOCUS       sequence-123093-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA for csr-1 locus in N. crassa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p426-SNR52p-gRNA.csr-1.Y-SUP4t
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    411..458
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact
      121 aatccaattg aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata
      181 cgataccccg catggaatgg gataatatca caggaggtac tagactacct ttcatcctac
      241 ataaatagac gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca
      301 tgtatatata tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg
      361 tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta
      421 ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa
      481 gcgctctgaa gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa
      541 aatattgcga ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc
      601 tgtgctatat ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa
      661 ctcgacctct acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta
      721 gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg
      781 tagtatatag agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat
      841 caacgctatc actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc
//
