LOCUS       sequence-123102-       10565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p53-L344A mutant tagged with mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Gaglia G, Guan Y, Shah JV, Lahav G
  TITLE     Activation and control of p53 tetramerization in individual living 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15497-501. doi: 
            10.1073/pnas.1311126110. Epub 2013 Sep 4.
  PUBMED    24006363
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1311126110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-09-17- 17"; volume: "110"; issue: "38"; pages: 
            "15497-501"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ashL344A-mCerulean
FEATURES             Location/Qualifiers
     source          1..10565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..1198
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FREANEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     protein_bind    complement(1200..1224)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1226..1228,1229..1231,1232..1945)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1271..1292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1532..1551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1879..1900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1946..1966)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1998..2354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1998..2188
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2020..2040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2205..2340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2389..3183
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2389..3183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2443..2462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3053..3072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3225..3813
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3278..3298)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3696..3707)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3888..4121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4193..4314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4230..4249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4284..4303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4333..4468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4500..4519)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4501..4519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4529..4545)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4538..4560)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4686..5141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4773..4792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4983..5004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5170..5274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5275..5343,5344..6135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5493..5512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6306..6894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6795..6814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7048..7065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7182..7203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7218..7223,7224..7241,7242..7248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7256..7272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7261..7283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7280..7296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7280..7296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7592..7772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7819..7944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8441..8674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8859..8903
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9196..9313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9353..9373
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        9374..10552
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9605..10543
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10500..10520
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    join(10558..10565,1..17)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtacaaaaaa gcaggcttca tggaggagcc gcagtcagat cctagcgtcg agccccctct
       61 gagtcaggaa acattttcag acctatggaa actacttcct gaaaacaacg ttctgtcccc
      121 cttgccgtcc caagcaatgg atgatttgat gctgtccccg gacgatattg aacaatggtt
      181 cactgaagac ccaggtccag atgaagctcc cagaatgcca gaggctgctc cccgcgtggc
      241 ccctgcacca gcagctccta caccggcggc ccctgcacca gccccctcct ggcccctgtc
      301 atcttctgtc ccttcccaga aaacctacca gggcagctac ggtttccgtc tgggcttctt
      361 gcattctggg acagccaagt ctgtgacttg cacgtactcc cctgccctca acaagatgtt
      421 ttgccaactg gccaagacct gccctgtgca gctgtgggtt gattccacac ccccgcccgg
      481 cacccgcgtc cgcgccatgg ccatctacaa gcagtcacag cacatgacgg aggttgtgag
      541 gcgctgcccc caccatgagc gctgctcaga tagcgatggt ctggcccctc ctcagcatct
      601 tatccgagtg gaaggaaatt tgcgtgtgga gtatttggat gacagaaaca cttttcgaca
      661 tagtgtggtg gtgccctatg agccgcctga ggttggctct gactgtacca ccatccacta
      721 caactacatg tgtaacagtt cctgcatggg cggcatgaac cggaggccca tcctcaccat
      781 catcacactg gaggatagct ctggaaacct actgggacgg aacagctttg aggtgcgtgt
      841 ttgtgcctgt cctgggagag accggcgcac agaggaagag aatctccgca agaaagggga
      901 gcctcaccac gagctgcccc cagggagcac taagcgagca ctgcccaaca acaccagctc
      961 ctctccccag ccaaagaaga aaccactgga tggagaatat ttcacccttc agatccgtgg
     1021 gcgtgagcgc ttcgagatgt tccgagaggc gaatgaggcc ttggaactca aggatgccca
     1081 ggctgggaag gagccagggg ggagcagggc tcactccagc cacctgaagt ccaaaaaggg
     1141 tcagtctacc tcccgccata aaaaactcat gttcaagaca gaagggcctg actcagacga
     1201 cccagctttc ttgtacaaag tgggaatggt gagcaagggc gaggagctgt tcaccggggt
     1261 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1321 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1381 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
     1441 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1501 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1561 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1621 ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatcagcg acaacgtcta
     1681 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1741 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1801 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     1861 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1921 cggcatggac gagctgtaca agtaacaact ttattataca tagttgataa ttcactggcc
     1981 gtcgagatcc ggactagtga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     2041 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2101 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2161 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2221 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2281 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2341 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     2401 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     2461 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     2521 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     2581 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     2641 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     2701 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     2761 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     2821 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     2881 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     2941 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     3001 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     3061 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     3121 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     3181 tgagcgacta gttaacgcgt cgaccagcta gctggccagt cgacaatcaa cctctggatt
     3241 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3301 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3361 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3421 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3481 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3541 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3601 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     3661 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3721 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3781 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta
     3841 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3901 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     3961 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4021 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4081 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     4141 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     4201 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4261 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4321 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4381 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     4441 aagtagtgag gaggcttttt tggaggccta ggcttttgcg tcgagacgta cccaattcgc
     4501 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     4561 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4621 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4681 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4741 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4981 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5041 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5101 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg
     5161 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5221 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5281 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5341 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5401 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5461 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5521 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5581 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5641 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5701 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5761 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5821 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5881 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5941 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6001 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6061 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6121 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6181 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6241 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6421 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7141 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7201 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7261 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     7321 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7381 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7441 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7501 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7561 gtatttaagt gcctagctcg atacaataaa cgggtctctc tggttagacc agatctgagc
     7621 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7681 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7741 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7801 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7861 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7921 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7981 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8041 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8101 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8161 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8221 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8281 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8341 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8401 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8461 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8521 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8581 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8641 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8701 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8761 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8821 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8881 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8941 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     9001 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     9061 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     9121 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
     9181 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9241 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9301 aattcaaaat tttatcgata agcttgatat caccatgatt acgccaagct atcaactttg
     9361 tatagaaaag ttgggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     9421 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     9481 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     9541 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     9601 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     9661 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     9721 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     9781 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     9841 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     9901 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     9961 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
    10021 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
    10081 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
    10141 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
    10201 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
    10261 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
    10321 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
    10381 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
    10441 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
    10501 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattca
    10561 agttt
//
