LOCUS       sequence-123115-         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 N863A nickase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX429 SpCas9 N863A nickase
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc
       61 tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc
      121 tcttcggagg gcacgttgtc gctcttgccc cgggccttgt cgcttctggt cagcaccttg
      181 ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tatggtccac atcgtagtcg
      241 gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag
      301 tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc
      361 tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc
      421 tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg
      481 tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc
      541 ttaatggcgg ggctgccggc cagattggca a
//
