LOCUS       sequence-123127-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retrovirus expressing GFP driven by EFS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: MSCV Puro-EFS:GFP
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(111..623,624..626,627..629))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     304..323
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     563..584
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(677..888)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
       61 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      121 tggcggatct tgaagttcgc cttgatgccg ttcttctgct tgtcggccat gatatagacg
      181 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      241 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      301 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      361 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
      421 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      481 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccagac
      541 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      601 gtgaacagct cctcgccctt gctcaccatg gtccgcttaa gctgtagcgc taatgtcggg
      661 tcccgcgtca cgacacctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg
      721 cgactactgc acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca
      781 cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga
      841 cccctccccc caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac
      901 cggagccact cgaaggcctc gggattccta ggaacagcgg ttttcaggca ccgggcttgc
      961 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     1021 cgagccgctc gt
//
