LOCUS       sequence-123129-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The artificial microRNA is designed for down-regulating expression 
            of ACD6 mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT79
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     87..103
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     88..104
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(544..560)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(586..610)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(618..634)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggaagttcat ttcatttgga gagaacacgg gggacgagct cgcccatcac aagtttgtac
       61 aaaaaagcag gctttcgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
      121 attcctgcag ccccaaacac acgctcggac gcatattaca catgttcata cacttaatac
      181 tcgctgtttt gaattgatgt tttaggaata tatatgtaga acagccttta gtctccatta
      241 ttcacaggtc gtgatatgat tcaattagct tccgactcat tcatccaaat accgagtcgc
      301 caaaattcaa actagactcg ttaaatgaat gaatgatgcg gtagacaaat tggatcattg
      361 attctctttg attaatggtg actaaaggcc gttctctctt ttgtattcca attttcttga
      421 ttaatctttc ctgcacaaaa acatgcttga tccactaagt gacatatatg ctgccttcgt
      481 atatatagtt ctggtaaaat taacattttg ggtttatctt tatttaaggc atcgccatgg
      541 ggggatccac tagttctaga gcggccgcca ccgcggtgga tctagaccca gctttcttgt
      601 acaaagtggt gatgggggat ccactagttc tagagtcctg ctttaatgag atatgcgaga
      661 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      721 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
      781 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
      841 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
      901 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
      961 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1021 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1081 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgag
//
